Morphological and physiological-biochemical variability of spore-forming bacteria isolated from the agrocoenosis of winter wheat

From an agrocoenosis of winter wheat (Triticum aestivum L.; phylloplane and rhizosphere the root system; typical chernozem, soil column measuring up to 40 cm), using classical microbiological methods, we had isolated bacteria characterized them according morphological features as representatives Gram-positive spore-forming Bacillus sp. genus. In earing-swelling phase grain, screening studies found non-pigmented forms colonies bacterial isolates, 19 which were classified colonial-morphological diversity R-type with diameter 7 13 mm. The analysis physiological condition cells populations isolates revealed technologic specificity parameters spore formation in different conditions incubation time (up 48–72 h). We observed 90.0% free spores axenic cultures early after 72 h cultivation no more than 10.0% prospores studied monoisolates stable morphologic traits. Isolates Н10 Н45 demonstrated ability grow higher temperatures (+37…+40 °С). According environmental рН, able рН ranging 4.5–8.0. Differential diagnostic testing that source carbon, acid, used arabinose, xylose, mannitol, glucose, galactose, fructose, maltose, sorbitol, glycerin, dextrin, starch, rhamnose dulcite (with development alkaline). There was active use mineral nitrogen: ammonium salt nitrates, aminoacids proteins. hydrolyzed casein, gelatin, litmus being reduced young during growth milk litmus. They also exerted catalase activity oxidase-positive. Biochemical API test system determined differed by a range fermentation carbohydrates, reduction nitrates. submerged fermentation, Н38 Н40 grew heightened temperature ranges (40 °С) for 48 (according fact development). Therefore, key biochemical traits, strains Н3, Н10, Н13, Н36, Н38, Н40, Н43, similar such reference strain B. subtilis 8A, identified sp., species subtilis.